Author: E. K. Sitepu, F. Sebayang , E.F. Zaidar , W. Ardilla , M. Antika , R.N. Sari , S. Perangin-angin and J.B. Tarigan

DOI: http://doi.org/10.31788/RJC.2023.1628216

Waste pineapple and passion fruit peels have potency as lipase enzyme sources. The combination of both peels with a ratio of 6:4 has the highest enzyme lipase activity after 3 days of fermentation and it has increased to 28.33 ± 0.0 U/ml after purification using the ammonium sulfate precipitation method. The response surface method design experiment was applied to determine the effect of the ratio of molar oil to methanol (1:3 – 1:9), reaction time (1 – 24 hours), and temperature (30 - 60C). The maximum biodiesel conversion of 82.7% could be achieved using the reaction condition of a ratio molar of 1:9, 13.65 hours reaction time, and a temperature of 46.5C for enzymatic transesterification of PO. Further, for bio-catalysis biodiesel production from WCO, the maximum conversion of 88% was achieved under reaction conditions of a ratio molar of 1:9, 14 hours reaction time, and a temperature of 48C.

Keywords: Pineapple, Passion Fruit, Waste Peels, Biodiesel, Lipase.