The purpose of the study was to determine the antioxidant potential, total phenol, and total flavonoid content in the nhexane fraction, ethyl acetate fraction, and water fraction of Eriobotrya japonica fruit. The research method for testing the antioxidant activity of the fruit of Eriobotrya Japonica was the DPPH (1.1-diphenyl-2-picryhydrazil ) method by UV-VIS spectrophotometry, while phenol and flavonoids contents were carried out by colorimetric method UV-Vis spectrophotometry. The determination of total phenol with Folin-Ciocalteu reagent and gallic acid as the standard, and the determination of total flavonoid content with AlCl3 reagent and quercetin as the standard. The results revealed that the IC50 values for the antioxidant activity of the n-hexane fraction, ethyl acetate fraction, and water fraction are 170.21, 68.33, and 351.95 g/mL, respectively. The total phenol concentration in the n-hexane fraction, ethyl acetate fraction, and water fraction, respectively, was 13.08, 95.34, and 15.24 mg GAE/g of material. The n-hexane fraction, ethyl acetate fraction, and water fraction contained 0.88, 12.65, and 2.6 mg QE per gram of material, respectively. Compared to the n-hexane and water fractions, the ethyl acetate fraction of Eriobotrya Japonica fruit had the highest quantities of total phenol and total flavonoid.