SYNTHESIS AND CHARACTERIZATION OFCINNAMALDEHYDE 4-HYDROXY METHYL PHENYL HYDRAZONE DERIVED FROM AN ISOLATED HYDRAZINE OF Agaricus bisporus: APPLICATION IN THE SPECTROPHOTOMETRIC DETERMINATION OF COBALT (II) IN BIOLOGICAL AND ENVIRONMENTAL SAMPLES
In this work, 4-hydroxy methyl phenyl hydrazine is isolated from Agaricus bisporus that was collected fromtheLambasingi region. Cinnamaldehyde-4-hydroxy methyl phenyl hydrazone (CHMPH) is then synthesised, characterised, and used for the spectrophotometric determination of cobalt (II). The hydrazone was created viaacondensation reaction between the mushroom extract and cinnamaldehyde, and it was identified by elemental analysis, IR, NMR, and mass spectroscopy. The CHMPH–Co(II) complex demonstrated a 1:1 stoichiometry, a linear Beer's law range of 0.029–0.29 μg/mL, a molar absorptivity of 8.4 × 10 4 L·mol -1cm-1 , and Sandell's sensitivityof 0.0007 μg/cm² under ideal conditions (pH 9.0, λmax 410 nm, and Triton X-100 5 %). High accuracy and precision(RSD < 2.5%) were shown by the good regression coefficient of 0.99985 and the detection and determination limitsof 0.0047 μg/mL and 0.0147 μg/mL, respectively. The suggested approach demonstrated findings similar to AASapproaches with errors within ±2% when used to determine Co (II) in a variety of matrices, including tea leaves, vitamin B12 tablets, alloys, and biological samples, including blood and urine. These results show that CHMPHis astraightforward, sensitive, and selective reagent for determining cobalt (II), with potential uses in analytical chemistry, biological, and environmental settings.
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