Author: V. Mittapelli, N. R. Parine, R. Merugu

DOI: http://doi.org/10.31788/RJC.2026.1919578

2-Heteryl and heteroalkyl substituted 4(3H)-quinazolinones have a captivating chemical structure with a groupof pharmacological activities. In the current investigation, we have undertaken the anti-inflammatory activity (in silico, in vitro and in vivo) followed by antioxidant studies of 1-(4-methylpheyl)-4-(3-methyl-4(3H)-quinazolinone-2-yl)-2- azetidinone (Compound-1) and N-(diethoxyphosphorylaminoacetyl)-N-(4-chlorophenyl)aminomethyl-3-methyl- 4(3H)-quinazolinone (Compound-2). Docking studies of both compounds for anti-inflammatory activitywasanalysed in silico using the docking server software. The Griess assay was executed to estimate the inflammatoryresponse of the compounds that inhibit the nitric oxide production. Both compounds showed low-to-moderateantioxidant activity. Compound-2 showed the highest anti-inflammatory activity and is comparable to Ibuprofen.

Keywords: 4(3H)-quinazolinone, Anti-inflammatory, Antioxidant, In vitro, In vivo, In silico, Docking Studies.