VALIDATED STABILITY-INDICATING HPTLC METHOD FOR QUANTIFICATION OF NORTRIPTYLINE
The examination was conducted using HPTLC plates of Silica gel 60F254 (20×10 cm, 250 μm) by using Toluene, Ethyl acetate, methanol, and Glacial acetic acid in the proportion of 4:4:1.6:0.4 v/v, which made up the mobile phase. After being prewashed with methanol, the plates were allowed to air dry before being activated for five minutes at 110°C. Samples were administered with a Camag micro syringe (150 nl/s) in 8 mm strips spaced 5 mmapart. At 26 ± 2 °C and 50 ± 5% RH, gradual development was carried out in a saturated twin-compartment chamber (20mindevelopment, 15 min saturation). The chromatogram was 80 mm in length. Using Camag winCATS (v1.4.5) inreflectance absorbance mode, with a deuterium lamp (190–400 nm), a 6 × 0.3 mm slit, and a scan speed of 20 mm/s, the plates are air-dried after development and checked at 279 nm. Peak area using linear regression served as thebasis for quantification. The correlation coefficient r 2= 0.00083 and linearity (200–600 ng/band) were demonstratedby the technique. The range of Nortriptyline recovery at 80%, 100%, and 120% was 101.45% to 101.97%. In order to conduct degradation investigations, Nortriptyline was refluxed with either 1N HCl or 1N NaOH for three hours at 80°C. After neutralisation, the solutions were diluted to 5000 ng/spot. Base degradation produced peaks at Rf 0.40, 0.45, 0.49, 0.58, and 0.62, whereas acid-degraded samples had peaks at Rf 0.42, 0.49, 0.53, 0.55, 0.62, and 0.82. Rf 0.39, 0.48, 0.57, and 0.73 were the locations of further degradation peaks that demonstrated the compound'svulnerability to both basic and acidic environments.
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