A sensitive spectrophotometric method has been developed for the determination of Maneb (manganese ethylenebisdithiocarbamate) by using a natural polymer chitin loaded with 1-(2’-pyridylazo)-2-naphthol (PAN). Maneb is quantitatively retained on the column and exchange the manganese present in it with PAN in the pH range 7.0-11.0 at a flow rate of 1-5 ml/min.The complex adsorbed on chitin is eluted from the column with 10 ml of dimethylformamide (DMF) and the absorbance of complexwas measured at 550 nm against a reagent blank. The molar absorptivity of the complex is 4.1 x 10⁴l mol^-1cm^-1 and Sandell’s sensitivity is 0.0064 µg cm^-2, respectively. The reproducibility of the method was checked by five replicateanalysis of 56.0 μg of maneb in 25 ml of final solution which gave a mean absorbance of 0.35 with RSD 1.2%. The method can be applied for the determination of Maneb in synthetic, commercial samples and in various foodstuffs.